Table 3 Effects of data quantity on microsatellite discovery based on genomic data (k-mer size = 65)
| Â | Amolops mantzorum | Quasipaa boulengeri | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Full | 1/2 | 1/4 | 1/8 | 1/16 | Full | 1/2 | 1/4 | 1/8 | 1/16 | |
Data in megabases (Mb) | 5200 | 2600 | 1300 | 650 | 327 | 4700 | 2400 | 1200 | 580 | 294 |
Microsatellite loci | 11,116 | 6511 | 3665 | 1929 | 1043 | 17,942 | 10,907 | 6317 | 3448 | 1883 |
Max RRLa (bp) | 64 | 64 | 60 | 60 | 60 | 66 | 66 | 65 | 65 | 63 |
Loci with RRL > 40 bp | 241 | 138 | 74 | 36 | 23 | 940 | 561 | 345 | 196 | 118 |
Mean RRL | 18.8 | 18.8 | 18.7 | 18.7 | 18.6 | 21.4 | 21.3 | 21.3 | 21.3 | 21.2 |
Dinucleotides | 8794 | 5171 | 2871 | 1517 | 836 | 11,527 | 7123 | 4215 | 2272 | 1289 |
Trinucleotides | 1640 | 943 | 550 | 300 | 145 | 4891 | 2832 | 1575 | 889 | 447 |
Tetranucleotides | 609 | 358 | 219 | 98 | 55 | 1361 | 854 | 460 | 266 | 140 |
Pentanucleotides | 55 | 29 | 18 | 11 | 5 | 127 | 76 | 54 | 18 | 6 |
Hexannucleotides | 18 | 10 | 7 | 3 | 2 | 36 | 22 | 13 | 3 | 1 |