Fig. 3

a. Expression patterns of mRNAs targets which presented a significant negative correlation to their respective miRNA expression during gestation. The heatmap was constructed using FPKM values and scaled for each mRNA target. The interactive heatmap can be accessed here. (http://rpubs.com/pouyadini/337845​). b. Comparative expression analysis between C24MC miRNAs (n = 21) and putative mRNA targets (n = 130) in equine CAM throughout pregnancy demonstrates a negative correlation in their overall expression pattern (r = − 0.78; P < 0.05). Correlation was calculated between the average expression pattern of C24MC-associated miRNA (n = 26 [no postpartum samples]) and the average expression pattern of predicted target mRNA (n = 16). Mean values ± standard error of the means are depicted