Fig. 5
From: Discerning novel splice junctions derived from RNA-seq alignment: a deep learning approach
Positive splice junctions tend to have high read support and contain the canonical flanking string. a Discrete proportions of negatives, positive semi-canonical splice junctions and positive canonical splice junctions from the classification results, given the average read support per sample. Splice junctions with average read support per sample more than 15 achieve a positive rate of around 88%. In contrast, for splice junctions with average read support per sample no more than 1, only 36% are identified as positive. There is a significant rise in the probability to obtain a positive splice junction with the increase of the average read support per sample. Around 99% positive splice junctions contain the canonical flanking string. b Cumulative proportions of positive semi-canonical and canonical splice junctions with the increase of the average read support per sample