Genome rearrangements in Escherichia coli during de novo acquisition of resistance to a single antibiotic or two antibiotics successively

Table 2 Primers used for PCR

Gene	Sequence (5′ - > 3′)	Annealing temperature (°C)
clpS-clpA	fw TGTGACAGATGTCGCTGATG	49
clpS-clpA	rv AAAGGCTTCCAGTTCCTGAC
fimA	fw AGCTGAATGATTGCGATACCA	49
fimA	rv GAAACCGGTTACTGCTGATTTG
yeaR	fw GAACGTACGGTATTCACCAGAT	49
yeaR	rv GAACGTACGGTATTCACCAGAT
dcuC-pagP	fw GCGAGCTACACCCACAATAA	49
dcuC-pagP	rv GTCATCCACTCATCTGCGTTAG
mgrB-yobH	fw GAAGAACCACCACCGATACAA	49
mgrB-yobH	rv CGCCATATCCGCTGAGTAATAA
clpX-lon	fw GTTGAATGAACTGAGCGAAGAAG	56
clpX-lon	rv TGCGCGACCAGCATAAT
oppB	fw CCAGAAGGTAGGGCAATGTT	56
oppB	rv CAATCATAGAGCCACGGGTAAT
cyoA	fw TAATGCCAGCGATCGTAACC	56
cyoA	rv CAACTCCGTGATGAACTCCTT

ISSN: 1471-2164