Fig. 4

Alternatively spliced pattern of five NlGrs. The data were verified by PCR with genomic DNA and cDNA as templetes. Bars represent exon sequence; lines represent intron sequences. Blue lines and black lines represent different splicing modes. Red lines in bars represent transmembrance domain. Splicing patterns include cassette exon skipping (NlGr1, NlGr21), alternative first exons (NlGr10), and alternative 5ʹ splice site (NlGr23). The histograms showed the different isoform relative usage from the RNA-seq data, blue bars represent blue line spliceosome