Fig. 8

Evolution of an idiomorphic AF-like cluster in T. inflatum. a Only two strains (567.84 and 824.70) harbor a complete cluster D, which is composed of at least two subclusters with independent evolutionary histories. Subcluster 1 (Box 1; blue) contains a PKS (7740) and three additional genes (7737, 7738, 7739) with homology to aflC, aflT, aflA, and aflB, respectively, of the AF mycotoxin cluster of Aspergillus flavus. Subcluster 2 (Box 2; red) contains two additional PKS genes (7749 and 7756) as well as a large number of accessory genes. All strains except 8044 harbor a pair of genes (7744 and 7745) that mark a boundary between the two subclusters. No homologs of genes from cluster D were detected in 8044. b Each subcluster shows a distinct pattern of co-diversification with genes found in other SMBGCs mined from a database of 340 fungal taxa. For subcluster 1, the four AF homologs are consistently clustered together but in different arrangements in AF-like clusters found in distinct Eurotiomycete (orange), Dothideomycete (green), and Sordariomycete (pink) taxa. The LD block found in the AF cluster across various Aspergillus spp. is shaded grey. Subcluster 2 shares four to seven genes with distinct SMBGCs found in other Eurotiomycete (orange), Dothideomycete (green), Leotiomycete (yellow), and Sordariomycete (pink) taxa. c Mauve alignment of the region of the chromosome containing cluster D and the syntenic regions in other strains revealed numerous genome rearrangements within and surrounding cluster D. The chromosome of each strain is shown as a line with genes marked in dark blue in a track below the line and colored shading above (forward) or below (inverted) the line indicating syntenic blocks relative to 714.70. Subclusters 1 and 2 are shown as blue and red boxes, respectively. A number of LTR TEs (green arrows) flank the cluster