Fig. 1

Schematic diagram of the pipeline for identification of duck lncRNAs. Clean reads were mapped and assembled according to the duck assembly using TopHat and Cufflinks. Putative lncRNAs were identified using five criteria: (1) filtered with duck protein-coding genes; (2) length > 200 nucleotides and ORF < 100 amino acids; (3) having no known protein domains; (4) low coding potential (CPC score < 0); (5) more than one exon