Fig. 4

RT-qPCR analysis on the effect of light and carS mutation on several putative stress-related genes. RT-qPCRs were carried out with the RNA samples from the wild type (WT), carS mutant SG39 (carS⁻) and complemented strain SG256 (C) used in the RNA-seq study. Darker bars: mycelia grown in the dark (D); paler bars: dark-grown mycelia exposed to light for 1 h (L). Data are referred to the data of the wild strain in the dark and show the mean and error of the mean of the two biological replicates. The unpaired t-test was applied to determine statistical differences between data from dark and light-exposed cultures and between wild type/C and carS⁻ cultures. Differences found to be statistically significant are indicated with asterisks according to P values of 0.0332 (*) and 0.021 (**)