Fig. 5
From: Isolation of blue-green eggshell pigmentation-related genes from Putian duck through RNA-seq
Expression levels of candidate genes by RT-qPCR and RNA-seq. The heat map shows the FPKM values for the seven selected candidate genes. Sample names are displayed below the column chart. Column height indicates the fold change in gene expression from the RT-qPCR results. RT-qPCR was performed with the primer sets in Table 3, and GAPDH was used as the reference gene. The error bars on each column indicate the SD based on three replicates. Different lowercase letters above the bars indicate statistically significant differences at P < 0.05 (one-way ANOVA, Duncan’s tests). Different letters indicate significant differences between groups, while the same letter represents no significant difference. The genes and gene_ids are as follows: carbonic anhydrase 4 (CA4, ncbi_101789643); prostatic acid phosphatase-like (ncbi_101805018); carbonic anhydrase 2 (ncbi_101802744); ovostatin (ncbi_101801176); CUNH2orf40 (ncbi_101802643); phospholipase A2 group XIIA (PLA2G12A, ncbi_101795972); polymeric immunoglobulin receptor-like (PIGR, ncbi_101802412)