Table 3 PCR primers used in this study
From: Isolation of blue-green eggshell pigmentation-related genes from Putian duck through RNA-seq
Primer | Sequence (5′-3′) |
|---|---|
GAPDH-F | AACGGTGACAGCCATTCCTC |
GAPDH-R | CCACCACACGGTTGCTGTAT |
Human C2orf40-F | CCGATGTGCAGCAGTGGTA |
Human C2orf40-R | TCCTCATCATAGTGGTGCTGGT |
CA4-F | AATCGGAGGTGGAGGTCTGA |
CA4-R | CCACAGCATCTTCTCTTATGTGG |
PIGR-F | CACTGCCGTTCATCTTCCTG |
PIGR-R | TCGATCAGGCCATACACCTG |
PLA2G12A-F | TGCAATCACCACGACAGATG |
PLA2G12A-R | CACAAGCCTGGACACTCTCTG |
Ovostatin-F | TTAGTGAGCGGCTACCAGCA |
Ovostatin-R | AAGGCAGTGAGCCAGGTGTT |
Carbonic anhydrase 2-F | CCACTCCACCACTGCTTGAA |
Carbonic anhydrase 2-R | CGCCAGTTGTCCACCATGT |
Prostatic acid phosphatase-like-F | GAGTGCTCAGGCGAGTCTTG |
Prostatic acid phosphatase-like-R | TGGCACTGTGTGAACTGGAA |
UDPase 2A2-F | CCTCCTACAAGGAAAATGCT |
UDPase 2A2-R | CGCATGACAAATTCAATCCA |
UGT1–1 like-F | ATGATCTTCTAGCTCACCCT |
UGT1–1 like-R | CTGTTTTCAAGGCGTTAGAC |
Protoheme IX farnesyltransferase-F | GAAAACAGGAATACGGCAAG |
Protoheme IX farnesyltransferase-R | GACTTAGGTTTTGCTCGTTG |
ALAS1-F | TTCCCATGGCAGATGACTAC |
ALAS1-R | TATCCATCACTGCTCCACAC |
BLVRA-F | AGAAAGAGGACCAGGGATGA |
BLVRA-R | CGCAAAATTCGCCATTTCTC |
GUSB glucuronidase-F | GCAGATATAAGCAGCATCGTC |
GUSB glucuronidase-R | ATACTGCACCTTTGCTTGTG |
Protoheme IX farnesyltransferase-2-F | CTTTCACGATGCTAAACGAG |
Protoheme IX farnesyltransferase-2-R | CACCGTGATGGATGTAGTAG |
EPRS glutamyl-prolyl-tRNA synthetase-F | GAGATGGGAAAGGTCATTGT |
EPRS glutamyl-prolyl-tRNA synthetase-R | ATTCGTGTCATCAAACCTCA |
EARS2-F | TATGCTTACTTGTGGGTCAG |
EARS2-R | GCTGCTGTACTTAGTCTCTC |
UROD-F | TTTTCTCTGACATCTTGGTGG |
UROD-R | CATGTAGGACATCAGCGTC |
CP-F | GGACCCATTATCAAGGCAGA |
CP-R | GTTCTTGCGGTAACTCACAC |
FECH-F | CTCACAAGTACTACATCGGG |
FECH-R | TACTCCACTTCATCTTTGGC |
COX15-F | CTGGGCTGGTACATGGTCAA |
COX15-R | GGTCTCGGGTAACTGGTGTC |
ALAD-F | AAACCTTTACCGGGATTTCT |
ALAD-R | TTCCTCAAAGATCCTCCTGT |
HMBS-F | ATTTGCCTGTTGCGTTTAGG |
HMBS-R | CCATTCTCCTTCAGCGTTTC |
FXN-F | TTTCTTTGAGGACCTAACCG |
FXN-R | AGAATACACCCAATTCCGTC |
Cytochrome c-type heme lyase-F | GGTTTCTGGTTCTTCTGACT |
Cytochrome c-type heme lyase-R | GCGCTCATGTAAAAACCATT |
UROS-F | TATGGGGGCCTAGTTTTCAC |
UROS-R | GTCTTTTCTCCTTGTGGGGA |
HEPH-F | GCAGACATGATCCCCAGTAA |
HEPH-R | GAAGGTCCATAGTCCCACTG |
CPOX-F | CACTAAACAGTGGTGGTTTG |
CPOX-R | AAGAATATGCCTCCAATCCC |
MMAB-F | CTGGAGCAGTGGATTGACA |
MMAB-R | TTCCCCTGCTTGTACTAACG |
HMOX1-F | ATACGTTGAAAGGCTCCACT |
HMOX1-R | TGGGCGATTTTCTTCAACAC |
HMOX2-F | AGTCTACACATGCTTGGCTT |
HMOX2-R | AAATTGAGTGCTCTTCGCTG |