Table 1 Candidate target and reference* genes in expression studies by qPCR in the shell gland of laying hens
Gene Name | Gene Symbol | Primer sequence (5′-3′) | Amplicon size (bp) | Ta °C | PCR Efficiency (%) | Accession No. | Reference |
|---|---|---|---|---|---|---|---|
Secreted phosphoprotein 1 | SPP1 | F-CCAGGAAGCTCATTGAGGATG R-GCTGTCGTTCTCTACGCTCT | 134 | 60 | 101 | NM_204535.4 | This study |
Proopiomelanocortin | POMC | F-TGGTGTTTTGGCGTGTGC R-CATCACGTACTTGCGGATGC | 115 | 65 | 105 | NM_001031098.1 | This study |
Calbindin | CALB1 | F: TTGGCACTGAAATCCCACTGA R: CATGCCAAGACCAAGGCTGA | 116 | 60 | 100 | NM_205513.1 | [71] |
Claudin 16 | CLDN16 | F- TACCTTGCTCATTGCAGGTCT R- GTGAGCAGGGACCCAGATAAG | 186 | 63 | 105 | XM_426702.3 | This study |
G protein subunit gamma 4 | GNG4 | F- CAGACCAATGCACAAGTTTCA R- GCCTCAAGTGGAAAGGTCAC | 243 | 63 | 97 | XM_004935468.2 | This study |
Potassium voltage-gated channel subfamily H member 1 | KCNH1 | F- AGAGGCAGAGATCCAGACGA R- GGTCTGATGTCCCAGACGTT | 160 | 63 | 93 | XM_015283863.1 | This study |
BPI fold containing family B, member 3 | BPIFB3 | F- CCATGCAACAAGTGCTGTCC R- AGCAGTTGCCACTGAGATCC | 234 | 63 | 94 | NM_001030861 | This study |
Rho related BTB domain containing 3 | RHOBTB3 | F- GACGTCGCATCTGTGATCC R- TCTTCCTTAGCTCGGCGTTA | 171 | 63 | 95 | ENSGALG00000014675 | This study |
Somatostatin II | SS2 | F- GCTCTTGGAGAGCTCAGACG R- GCACTAGCAGGAGGTGAAGG | 160 | 65 | 97 | NM_204455.1 | This study |
Otopetrin 2 | OTOP2 | F- GGAGCAAGCAATTGCCCAAA R- CGCTGCTTTGCTGCCTG | 202 | 63 | 99 | XM_003642368.3 | This study |
Klotho beta | KLB | F- GAGCAATACGGGGGATGGAA R- GCATGAGCCTTGATCAGATTGT | 224 | 61 | 101 | XM_003641245.3 | This study |
Glycoprotein hormones, alpha polypeptide | CGA | F- GTCCAGAGTGCAAGCTAGGG R- GCTACACAGCACGTTGCTTC | 166 | 63 | 105 | :NM_001278021 | This study |
GATA-binding factor 3 | GATA3 | F- CAGAAGGCAGGGAGTGTGTAA R- GCTGCAGACAGCCTTCTCT | 157 | 63 | 100 | NM_001008444 | This study |
TYRO3 protein tyrosine kinase | TYRO3 | F- GTGCAGTGCAGCAATGAGAT R- CAGCCTGTATCCCAGGACAT | 186 | 61 | 98 | NM_204627 | This study |
Matrix metallopeptidase 13 | MMP13 | F- AGCTCTATGGTGCTGGAGAC R- CCTGTCCTTGAAGACCAGCAT | 128 | 63 | 100 | NM_001293090 | This study |
Gap junction protein, alpha 8 | GJA8 | F- CTGCTATGATGAGGCCTTCC R- GGCAGCTTCTCTTCATCCAC | 186 | 63 | 98 | NM_204997.1 | This study |
Carbonic anhydrase 9 | CA9 | F- CTCAGTGACAGCAGCAGGA R- TGGCAAAGAGCACTCCGAAT | 80 | 63 | 99 | XM_004937157.2 | This study |
Cytochrome P450 family 7 subfamily A member 1 | CYP7A1 | F- AGGAGGCAATGAGGCTATCG R- TGAGTGTCAAGGGATCAGCA | 171 | 63 | 96 | NM_001001753.1 | This study |
Galactose-3-O-sulfotransferase | GAL3ST2 | F- TGCTTCGAGGACTACCAAAAA R- TGGGTCTTGAGGAACATGACG | 177 | 61 | 96 | NM_001277431.1 | This study |
TATA-Box Binding Protein | TBP* | F: TAGCCCGATGATGCCGTAT R: GTTCCCTGTGTCGCTTGC | 147 | 61 | 97 | NM_205103 | [72] |
Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide | YWHAZ* | F- TTGCTGCTGGAGATGACAAG R- CTTCTTGATACGCCTGTTG | 61 | 60 | 104 | NM_001031343.1 | [73] |