Fig. 7

Validation of circRNAs by experimental and sequencing. Note: (a), agarose gel electrophoresis test for divergent primers and convergent primers amplify circRNAs; The brightest DNA marker is 200 bp. cc: convergent primers for cDNA; cg: convergent primers for genomic DNA; dc: divergent primers for cDNA; dg: divergent primers for genomic DNA. b, Sanger sequencing confirmed the back-splicing junction of circRNAs; (c), RT-qPCR validation of nine differentially expressed circRNAs in three types of granulosa cell. TPM: Transcripts Per Million; RQ: relative expression; Graph number of a, b, c, d, e, f, g, h, i represent circRNAs: circRNA_8: 6369673|6,402,248, circRNA_8: 6369673|6,422,097, circRNA_8: 6384248|6,402,248, circRNA_26: 4587446|4,664,529, circRNA_3: 66399375|66,402,723, circRNA_20: 2426006|2,445,902, circRNA_14: 84666291|8,458,456, circRNA_12: 9000575|9,001,598, circRNA_23: 5084518|5,086,817, respectively. SYF: small yellow follicle; F6: the smallest hierarchal follicles; F1: the largest hierarchal follicle; G: granulosa cell.