Table 1 Validation of differentially expressed genes related to cell wall metabolism, transcription factors and receptor-like kinases in leaves of drought-stressed sweet orange (Citrus sinensis) grafted on ‘Rangpur’ lime
ID citrus | Gene description | RNAseq fold change (P ≤ 0.001) | qPCR log2 fold change* |
|---|---|---|---|
Cell wall metabolism | |||
 Orange1.1g025810m | Expansin-Like B1 | 36.764 | 3.716 |
 Orange1.1g013402m | Polygalacturonase (pectinase) | 14.367 | 0.539 |
 Orange1.1g030669m | Arabinogalactan protein 1 | 3.787 | 1.406 |
 Orange1.1g047288m | Pectinesterase inhibitor 6-like | −4.516 | −0.579 |
 Orange1.1g029350m | Proline-Rich Protein 4 | −6.134 | 0.219 |
 Orange1.1g009682m | Pectinesterase inhibitor 54-like | −11.369 | 0.205 |
 Orange1.1g025025m | Expansin A4 | −12.863 | −1.552 |
 Orange1.1g007089m | β-Xylosidade 1 | −14.818 | 0.317 |
 Orange1.1g011204m | Pectin lyase-1 like | −77.398 | −2.640 |
Transcription factor | |||
 Orange1.1g024111m | bHLH BEE 3-like | 12.023 | 5.925 |
 Orange1.1g025097m | WRKY transcription factor 40 | 14.04 | 3.412 |
 Orange1.1g021910m | NAC transcription factor 2 | 10.171 | 2.673 |
Receptor-like kinase | |||
 Orange1.1 g031436 | Wall-associated receptor kinase-like 8 | 7.145 | −2.482 |
 Orange1.1 g026214 | Wall-associated receptor kinase-like 2 | 8.891 | 1.386 |
 Orange1.1 g041671 | Wall-associated receptor kinase 2 | 4.977 | −0.477 |