Fig. 1

Histopathological assessment of genital tract of mice infected once with Chlamydia. a. Representative image of the endometrium from an uninfected mouse. A single layer of columnar epithelia (arrow), with basally located nuclei lining the endometrium. The underlying lamina propria has scattered spindled cells, some capillaries, and occasional leukocytes. Hematoxylin and eosin (HE) stain. Scale bar = 20 μm. b and c. Representative image of uterine endometrium with increased presence of eosinophils after the first and second weeks of infection. The lamina propria is mildly hypercellular, with loosely arranged spindled stromal cells and increased numbers of eosinophils around an endometrial gland (Gl). HE stain. Scale bar 20 μm. d-f. Representative images of inflammed uterine endometrium with necrosis after 4 weeks of infection. d. The uterine lumen (asterisks) is filled with necrotic cellular debris. The mucosa has widespread epithelial apoptotic necrosis (arrowheads). The lamina propria is moderately hypercellular, with increased numbers of neutrophils, eosinophils, and few lymphocytes (arrows). HE stain. Scale bar 20 μm