Fig. 1

Hundreds of lncRNAs are dysregulated in NHL compared to normal B cells. a Schematic depicts collection, flow cytometry purification, and ‘omics profiling of malignant and normal B lymphocytes from NHL patients and healthy volunteers [21]. b Diagram of NHL lncRNA discovery pipeline. RNA-seq data was analyzed using a de novo processing pipeline to enable identification of novel transcripts (Cufflinks). Novel RNA transcripts were merged with annotated transcripts (Cuffmerge). c Volcano plot highlights lncRNA transcripts with significantly different expression in NHL tumor samples compared to normal B cells (red). Relative expression of lncRNA transcripts shown in log2 fold change expression (FPKM) versus –log10 adjusted p value (FDR, Benjamini&Hochberg) for NHL:normal B cells. d Data as in C, with different types of lncRNAs highlighted in different colors (red: annotated lncRNAs, blue: intergenic lincRNAs, green: novel (not annotated) lncRNAs