Fig. 5

Comparison of differential gene expression between CCI and naïve groups in male and female rats. a Schematic diagram of experiment. Male and female rats were randomly assigned to the naïve group or receive CCI. RNA-seq performed on ipsilateral L4-L6 DRGs from each animal. Differentially expressed genes (DEG) defined as genes expressed after CCI versus naïve with a |log₂FC| > 0.5 and an FDR < 0.05. b Principal component analysis of male and female rats from the naïve and CCI groups. c Heatmap that shows the log₂FC of the 200 most differentially expressed genes between the CCI and Naïve groups in female and male rats. d Venn diagram shows the number of genes that are differentially expressed in males (orange) and females (green). Log₂FC expression between the CCI and naive males (x-axis) and females (y-axis). Threshold of |log₂FC| > 0.5(dashed lines) with an FDR < 0.05 designates DEGs in female rats only (green), male rats only (orange), and in both male and female rats (brown). DRG dorsal root ganglia, CCI chronic constriction injury, FC fold change, FDR false discovery rate