Table 4 Putative secreted effectors up-regulated during infection

Sscle03g028510

12.19

10.01

6.15

−4.98

Possible virulence factor characterized in B. napus infection (Ssv263) [15].

Sscle07g055350

8.03

8.87

10.83

–

Contains a CFEM domain (pfam05730). Found to be up-regulated in Brassica infection [24]

Sscle05g045060

8.4

8.84

9.09

–

Identified as a predicted effector by Derbyshire et al. 2017 [58].

Sscle06g048920

8.68

9.33

8.24

–

Identified as a predicted effector by Derbyshire et al. 2017 [58].

Sscle06g055310

7.72

8.02

7.76

–

Related to Immunoglobin E (IgE) binding proteins in Aspergillus fumigatus (~ 40% identity). Homologue to Sscle11g080900 (99% identity).

Sscle11g080900

7.25

7.64

7.37

–

Related to Immunoglobin E (IgE) binding proteins in Aspergillus fumigatus (~ 40% identity). Homologue to Sscle06g055310 (99% identity).

Sscle16g107670

6.97

6.68

7.15

–

A gene containing a cerato-platanin domain (pfam07249) which was characterized as Sscp1 and found to be important in full virulence of S. sclerotiorum [62]

Sscle08g068200

6.29

7.05

6.52

–

Contains two chitin recognition domains (pfam00187) and a fungal cellulose binding domain (pfam00734).

Sscle01g003850

4.96

6.03

7.48

1.98

Characterized as a virulence gene in S. sclerotiorum (Ssssvp1). Binds with a subunit of the cytochrome b-c1 complex of the mitochondrial respiratory chain and prevents subcellular localization into the mitochondria, subsequently leading to plant cell death [18].

Sscle12g090490

5.17

5.78

5.9

–

A necrosis and ethylene-inducing peptide (SsNEP2) found to induce cell death in plants [16].

Sscle05g041050

3.43

5.52

7.66

3.19

Identified as a predicted effector by Derbyshire et al. 2017 [58].

Sscle03g024000

5.15

4.72

4.81

–

Shares 59% identity with Colletotrichum hingginsianum effector candidate 91 (CHEC91) [104] and a Colletotrichum incanum gene categorized as a “hypersensitive-response inducing protein.”

Sscle08g064180

4.29

5.26

4.97

–

Identified as a predicted effector by Derbyshire et al. 2017 [58].

Sscle03g022790

2.69

5.47

5.7

–

Contains a fungal hydrophobin domain (pfam06766). Possibly involved in mediating the interaction of fungal tissue and the plant. Often connected to the outside of fungal conidia or on the hyphal wall.

Sscle11g082980

6.22

3.86

3.57

–

A non-aspartyl acid protease (acp1) found to be expressed during S. sclerotiorum infection of sunflower cotyledons. [38]

Sscle06g049430

6.57

3.11

3.72

–

A secretory Rhs repeat-containing protein (Ss-Rhs1) found to be important in sclerotial development and hyphal infection. [87]

Sscle04g040080

3.49

3.76

5.08

–

Identified as a predicted effector by Derbyshire et al. 2017 [58]. Contains a protein domain of unknown function (DUF3129; pfam11327).

Sscle08g063910

3.95

3.14

4.18

–

Found to be up-regulated in Brassica infection [24].

Sscle07g061770

–

4.19

5.11

–

Identified as a predicted effector by Derbyshire et al. 2017 [58].

Sscle10g075140

–

5.37

3.72

–

Identified as a predicted effector by Derbyshire et al. 2017 [58].

Sscle06g052360

3.52

2.63

2.82

–

Identified as a predicted effector by Derbyshire et al. 2017 [58].

Sscle15g106630

4.34

2.76

1.76

−1.82

Found to be up-regulated in Brassica infection [24].

Sscle16g111080

3.77

2.73

1.71

−1.56

A putative chorismate mutase which is expressed in S. sclerotiorum infection of Brassica and is predicted to interfere with salicylic acid (SA) signaling in infected plants [88].

Sscle05g042890

2.31

2.76

2.51

–

Contains a Lytic polysaccharide mono-oxygenase, cellulose-degrading domain (LPMO-10; pfam03067), related to cellulose degradation. This domain was previously characterized as a chitin binding domain.

Sscle01g000190

2.38

2.4

2.61

–

Contains a pyrroline-5-carboxylate reductase domain (pfam14748). Possibly involved in proline biosynthesis.

Sscle16g107890

3.21

3.58

–

–

Identified as a predicted effector by Derbyshire et al. 2017 [58].

Sscle07g062010

1.38

2.82

2.29

–

Contains a glycosyl hydrolases, family 18 (GH18) type II chitinase. Found to be up-regulated in B. napus infection [24]

Sscle07g058780

1.44

2.2

2.18

–

Found to be up-regulated during Arabidopsis infection [105].

Sscle04g035160

–

2.69

3.1

–

Identified as a predicted effector by Derbyshire et al. 2017 [58].

Sscle13g096120

3.42

–

2.07

–

Identified as a predicted effector by Guyon et al. 2014 [22].

Sscle08g065470

2.24

1.56

1.66

–

Contains an amidase domain (pfam01425). Identified as a predicted effector by Guyon et al. 2014 [22].

Sscle06g054400

–

1.72

3.11

–

Identified as a predicted effector by Derbyshire et al. 2017 [58].

Sscle10g078230

–

3.05

1.71

–

A putative salicylate hydrolase. Contains salicylate 1-monooxygenase (TIGR03219) and FAD binding (pfam01494) domains.

Sscle08g064590

–

2.17

2.53

–

Identified as a predicted effector by Derbyshire et al. 2017 [58].

Sscle12g088370

–

1.43

3.16

2.6

Identified as a predicted effector by Guyon et al. 2014 [22].

Sscle06g054180

–

–

4.37

4.53

Contains a Lysin motif (smart00257). Found to be up-regulated in Brassica infection [24].

Sscle07g057820

1.52

1.27

1.31

–

A likely subtilisin-like serine protease. Also contains a peptidase inhibitor domain (pfam05922), suggesting that it may be at the very least a temporary protease inhibitor.

Sscle06g050820

2.08

–

1.96

–

Identified as a predicted effector by Derbyshire et al. 2017 [58].

Sscle07g062060

1.19

1.32

1.46

–

Identified as a predicted effector by Derbyshire et al. 2017 [58].

Sscle01g007640

1.2

1.23

1.22

–

A serine protease inhibitor containing a Peptidase inhibitor I9 domain (PF05922) which was expressed during infection of Arabidopsis [22].

Sscle14g100310

–

–

3.56

3.54

Identified as a predicted effector by Derbyshire et al. 2017 [58].

Sscle01g005870

–

–

3.12

1.79

Putative Choline dehydrogenase or related flavoprotein (COG2303). Potentially involved in lipid transport or metabolism. 36% identity to Sscle06g048860.

Sscle08g066850

–

–

2.4

3.22

Contains chitin recognition domain (pfam00187) and GH18 domains containing both catalytically active and inactive chitinase-like proteins (cd02872).

Sscle14g098710

–

2.3

–

–

. Contains a Common central domain of tyrosinase (pfam00264).

Sscle08g068500

–

–

2.06

–

Characterized as Ssitl and found to suppress JA signaling pathways

Sscle01g000660

2.19

–

–

–

Identified as a predicted effector by Derbyshire et al. 2017 [58].

Sscle04g036550

2.15

–

–

–

Identified as a predicted effector by Derbyshire et al. 2017 [58].

Sscle01g004200

–

–

2.16

–

Homologue of the predicted effector 5 protein discovered in Venturia inaequalis (Thakur et al. 2012).

Sscle14g098880

–

–

1.94

2.99

Contains a ricin type beta-trefoil lectin domain (PF00652). This domain is common in the formation of toxins, but is also known to be involved in signal transduction.

Sscle04g038020

–

–

1.84

5.3

Contains a CyanoVirin-N Homology (CVNH) domain (pfam08881). Experimentally confirmed to be essential for full virulence [106].

Sscle12g090380

–

1.84

–

–

Identified as a predicted effector by Derbyshire et al. 2017 [58].

Sscle06g050280

–

–

–

5.04

Contains a CFEM domain (pfam05730) often associated with fungal pathogenesis. Up-regulation was observed in B. napus infection, but not during the infection of several other host plants [22, 24].

Sscle06g048860

–

–

–

3

Putative Choline dehydrogenase or related flavoprotein (COG2303). Potentially involved in lipid transport or metabolism. 36% identity to Sscle01g005870.

Sscle08g063080

–

–

–

1.87

Putative tripeptidyl peptidase shown to be up-regulated during infection of B. napus [24].

.Sscle06g053560

–

–

–

2.5

Shares 72% identity with a cuticle-degrading serine protease found in Marssonina brunnea. Also contains a peptidase inhibitor domain (pfam05922).

Sscle02g012940

–

–

–

2.68

Identified as a predicted effector by Derbyshire et al. 2017 [58].

Sscle01g008950

–

–

–

2.75

Identified as a predicted effector by Derbyshire et al. 2017 [58], but found to be strongly up-regulated in developing apothecia. The relationship between the gene and virulence is considered suspect [107].