Fig. 6

Overexpression and silencing of GmNAC42–1 in soybean hairy roots. a Gene expressions in WGE-treated Williams 82 hairy roots undergoing RNAi silencing of GmNAC42–1. b Gene expressions in WGE-treated hairy roots overexpressing GmNAC42–1. c Gene expressions in mock-treated hairy roots overexpressing GmNAC42–1. Measurements were 24 h after treatment by qRT-PCR. ^aSignificantly greater and ^bsignificantly less than control, paired students t-test (P < 0.01). d Amounts of phytoalexins and constitutive isoflavonoids in soybean hairy roots undergoing RNAi silencing of GmNAC42–1 24 h after treatment with WGE or H2O. e Metabolite amounts from hairy roots overexpressing GmNAC42–1. Different letters show significant differences by single factor ANOVA, Tukey post hoc test, P < 0.01