Table 2 List of qPCR primers used to validate the expression results from the RNA-seq analysis
From: Gene expression of Hanwoo satellite cell differentiation in longissimus dorsi and semimembranosus
Gene | Sequence (5′-3′) |
|---|---|
RPS9 (accession no. DT860044) | Forward - GAGCTGGGTTTGTCGCAAAA |
Reverse - GGTCGAGGCGGGACTTCT | |
Taqman probe -6FAM-ATGTGACCCCGCGGAGACCCTTC-TAMRA | |
Myogenin (accession no. AF091714) | Forward - AGAAGGTGAATGAAGCCTTCGA |
Reverse - GCAGGCGCTCTATGTACTGGAT | |
Taqman probe - 6FAM-CCCAACCAGAGGCTGCCCAAAGT-TAMRA | |
Myo D (accession no. AB110599) | Forward - AGGCCTTCGAGACGCTCAA |
Reverse - TGGCGTTGCGCAGGAT | |
Taqman probe - 6FAM-CGCTGCACGTCTAGCAACCCAAACC-TAMRA | |
Myf 5 (accession no. NM_174116) | Forward - GGCTTTCGACACGCTCAAG |
Reverse - CATTCCTGAGGATCTCCACCTT | |
Taqman probe - 6FAM-TGCACCACGACCAACCCTAACCAGA-TAMRA | |
MYF6 (accession no. NM_181811) | Forward - GGAGGTGGTGGAGAAGTAACTCA |
Reverse - GCAGGGAGGGTGGGATCTT | |
Taqman probe - 6FAM-TCCGGACGTTCTCCACGGAGCA-TAMRA | |
IGF1 (accession no. NM_001077828) | Forward - GGTGAAGATGCCCATCACATC |
Reverse - GCTGGTGAAGGCGAGCAA | |
Taqman probe - 6FAM-TCCTCGCATCTCTTCTATCTGGCCCTG-TAMRA | |
PAX3 (accession no. NM_001206818) | Forward - GGACAGCAGCTCTGCCTACTG |
Reverse - GAGGCACAAAGCTGTCTGTATAGC | |
Taqman probe - 6FAM-CTCCCCAGCACCAGGCATGGA-TAMRA | |
HOXC11 (accession no. NM_001192873) | Forward - GCACTTACTACGTGCCTGAGTTCTC |
Reverse - TTGGGCCGGGTAGGGATA | |
Taqman probe - 6FAM-CCCCAGGCCCCCTCTCGTCAG-TAMRA | |
SIM2 (accession no. XM_015462213) | Forward - CGAAGCTGAGAGCAAACCCTTA |
Reverse - CGCACTCCAGTTTGTCCATTT | |
Taqman probe - 6FAM-CCCGCACAGCAGTACGGCTCG-TAMRA | |
HOXC8 (accession no. XM_002687232) | Forward - TCGCACCACGTCCAAGACT |
Reverse - TCTGCTGGTAGCCCGAGTTG | |
Taqman probe - 6FAM-CTTCCACCACGGCACCTCGGG-TAMRA | |
HOXB9 (accession no. NM_001191186) | Forward - GGCAACCCCAGTTCCTCACT |
Reverse - TCCCCGGGTGACTTTGG | |
Taqman probe - 6FAM-CCAACCTGCCTGTTCCTTCCCACA-TAMRA | |
ZIC2 (accession no. NM_001206366) | Forward - CAAGCAAGAGCTCATCTGCAAGT |
Reverse - TGAAAGTTTTGTTGCAGCTCTTCT | |
Taqman probe - 6FAM-ATCGACCCCGAGCAGCTGAGTAACC-TAMRA | |
ZIC4 (accession no. XM_586391) | Forward - GCGCCTTTGCTCCAAAACT |
Reverse - ACGTGCTCCACGGTGACAT | |
Taqman probe - 6FAM-TCAGCACCATGCACGAGCTGGTC-TAMRA | |
TBX4 (accession no. NM_001192193) | Forward - GATGTTCCCCAGCTACAAGGTAA |
Reverse - CGATGTCGATCAGCAGGATGT | |
Taqman probe - 6FAM-CACGGGCATGAACCCCAAGACC-TAMRA | |
HOXB4 (accession no. NM_001078114) | Forward - AGTGTTTTGGCCACGGTAACA |
Reverse - CGGCCCCAAGGTGGAA | |
Taqman probe - 6FAM-CTTCCCCCTCCATGCCCGTTCA-TAMRA |