Fig. 1

Hybridization-based enrichment enhances sensitivity of HTS for viral detection from complex samples. Known concentrations of IFV genomic RNA (gRNA) were spiked into complex matrices. Samples were split into two parts and processed in parallel via unbiased, shotgun sequencing or target enrichment sequencing in pools of four. a IFV was spiked into bat guano at increasing concentrations to simulate environmental-type samples. Shown here is the percentage of IFV-specific reads. b Increasing concentrations of IFV gRNA were spiked into total RNA derived from MERS-CoV cell culture lysate. MERS-CoV genomic material was present at a constant, high level amongst all samples. The average percentage of IFV and MERS-CoV virus-specific reads derived from three biological replicates is shown. Black bars denote standard error of the mean for each sample