Fig. 4

Eukaryotic Initiation Factor (EIF) expression across embryo development and the individual 8-cell blastomeres a) Heat Map to represent the 45 EIFs expressed constitutively within at least one oocyte or embryonic stage. Mas5 flags with 75% expression within a specific developmental stage are classed as expressed. b) Schematic indicating differentially expressed EIFs (Benjamini-Hochberg FDR ≤ 0.05) between the 4-cell embryo, 8-cell embryo and blastocyst. c) Frozen robust multi-array analysis (fRMA) was used to define absolute expression by comparison to publically available microarray datasets within R and an expression barcode was defined for each 8-cell blastomere. The heat map represent EIFs expression within individual 8-cell blastomere expression barcode data, genes with a score of ‘1’ are present and ‘0’ are absent. Half of the EIFs associated with EGA are expressed in all 8 blastomeres, the other half are variably expressed, with blastomere B1 expressing the full complement (10/10), while B2 expressed the fewest (3), with B8 expressing 5/10