Fig. 1

Effect of single and combined PPARα and CREB3L3 deficiency on metabolic parameters. PPARα−/−, CREB3L3−/− and combined PPARα/CREB3L3−/− mice were subjected to a 16-h fast. a Plasma triglycerides. WT, n = 9; CREB3L3−/−, n = 11; PPARα−/−, n = 7; PPARα/CREB3L3−/−, n = 6. b Plasma non-esterified fatty acids (NEFA). WT, n = 9; CREB3L3−/−, n = 11; PPARα−/−, n = 8; PPARα/CREB3L3−/−, n = 6. c Plasma β-hydroxybutyrate. WT, n = 9; CREB3L3−/−, n = 11; PPARα−/−, n = 8; PPARα/CREB3L3−/−, n = 6. d Hepatic triglycerides. WT, n = 7; CREB3L3−/−, n = 9; PPARα−/−, n = 6; PPARα/CREB3L3−/−, n = 6. e Plasma Fibroblast Growth Factor 21. WT, n = 5; CREB3L3−/−, n = 8; PPARα−/−, n = 4; PPARα/CREB3L3−/−, n = 4. Error bars represent SEM. Asterisk indicates significant effect of CREB3L3 deficiency in wild-type mice (blue vs. white bar) and in PPARα mice (purple vs red bar) according to Student’s t-test (*P < 0.05, **P < 0.01, ***P < 0.001). Pound sign indicates significant effect of PPARα deficiency in wild-type mice (red vs. white bar) and in CREB3L3 mice (purple vs. blue bar) according to Student’s t-test (#P < 0.05, ##P < 0.01, ###P < 0.001)