Fig. 2
Largely independent effect of PPARα and CREB3L3 deficiency on hepatic gene expression in the fasted state. a Volcano plot showing the relation between mean signal log ratio (2log[fold-change], x-axis) and the -10log of the IBMT P-value (y-axis) for the comparison between wild-type mice and PPARα−/− mice, CREB3L3−/− mice and combined PPARα/CREB3L3−/− mice after a 16-h fast. b Number of genes meeting significance criteria (fold change<− 1.2 or > 1.2 and IBMT P < 0.001) for the comparison between wild-type mice and PPARα−/− mice, CREB3L3−/− mice and combined PPARα/CREB3L3−/− mice after a 16-h fast. Principle component analysis (c) and hierarchical clustering (d) of transcriptomics data from livers of wild-type, PPARα−/−, CREB3L3−/−, and combined PPARα/CREB3L3−/− mice after a 16-h fast. Distance criteria are based on Pearson correlation with average linkage. An IQR (Inter Quartile Range) filter of 0.5 was applied. e Hierarchical biclustering of samples and genes visualized in a heatmap. Data were centered on wild-type mean. Clustering was done based on Pearson correlation with average linkage. Red indicates upregulated, blue indicates downregulated. The region in the heatmap that is suggestive of synergistic regulation by PPARα and CREB3L3 deficiency is indicated by a grey arrow