Fig. 2From: Reliable multiplex sequencing with rare index mis-assignment on DNB-based NGS platformLibrary preparation workflows. a “standard PCR-based WGS”-like library; (b) PCR-free library; (c) two-step PCR library. Pooling after each step, indicated by red arrows, is examined for different library preparation strategies. Gray rectangle, adapter; colored rectangle, unique index assigned to a particular sample; gray vertical lines, unique sample index; white rectangle, UIDBack to article page