Fig. 3

Muscle function varies with genetic background in C. elegans ILs constitutively expressing α-synuclein. Boxplots of the number of pharyngeal pumps over a 30 s period in worms at (a) 48 and (b) 72 h after recovery from L1 arrest. Black dots represent outliers (two times interquartile range). Analysis shows no significant effects at 48 h. At 72 h however, pharyngeal pumping is affected by α-synuclein expression, by the genetic background and by the interaction of α-synuclein expression with the genetic background (ANOVA: aS, p < 3e-16, Genotype, p < 3e-10, aS x Genotype, p < 0.004; Tukey HSD: N2 p = 0.93; JU1511 p < 5e-4; JU1926 p < 2e-5; JU1931 p = 0.54; JU1941 p < 1e-8). c Boxplots of the activity rates of worms in liquid, with analysis indicating that locomotion pumping is affected by α-synuclein expression, by the genetic background and by the interaction of α-synuclein expression with the genetic background (ANOVA: aS p < 1.953e-12, Genotype p < 4.4e-13, aS x Genotype p = 0.0040; Tukey HSD: N2 p = 1.00; JU1511 p = 2.4e-6; JU1926 p = 0.0016; JU1931 p = 1.3e-3; JU1941 p = 0.51). Significant differences between genotypes are indicated by stars