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Fig. 3 | BMC Genomics

Fig. 3

From: Development of drug-inducible CRISPR-Cas9 systems for large-scale functional screening

Fig. 3

In vivo genome editing using the DOX-inducible sgRNA expression construct. (a) Schematic diagram of testing the DOX-inducible sgRNA expression vector in bone marrow chimeric mice. (b) Representative flow cytometry histograms showing DOX-inducible Cd44 knockout in splenic cells. (D) DOX-inducible Cd44 knockout in CD11b+, CD11c + and CD19+ cells in spleen. (d) DOX-inducible Cd44 knockout in CD11b + and CD19+ cells in bone marrow. (e) DOX-inducible Cd44 knockout in CD11b + and CD19+ cells in peripheral blood. Individual animals and mean (c-e) are shown with five mice per group. P values were derived from t tests: *P < 0.05; **P < 0.01; ***P < 0.001; NS, nonsignificant

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