Fig. 2

Detection of antisense ncRNAs and mapping of their TSS. a Detection of SP119_SR097 antisense to recU and sequence alignment showing the conservation of the corresponding promoter among streptococci (the extended − 10 box sequence and the TSS that have been characterized are underlined). b Detection of SP119_SR049 potentially regulating expression of clpL in S. pyogenes. The sequence reads mapped to the genome of strain S119, in conditions of dRNA-seq: strand-specific sequencing of transcript 5′ ends with (TAP+) and without (TAP-) TAP treatment, and strand-specific RNA-seq, are visualized by using IGV. On the schematic view of the gene organization, the protein coding genes annotated on the (+) and (−) strands (Str) are indicated by red and blue large arrows respectively. TSSs are depicted as small arrows (red and blue for plus and minus strands respectively). The identified transcripts are shown by thin arrows