Fig. 5

ALFA biotype plots (raw and normalized) for RNA-Seq data on Homo sapiens samples (public data available on NCBI: SRP058036). This dataset is part of a research work where ribosomal RNA depletion is compared between adult and fetal tissues [18]. This study reported that a large portion of transcripts with mitochondrial ribosomal origin was observed, in particular in colon, heart and kidney samples. For clarity, only lung (SRR2014234 and SRR2014235) and heart (SRR2014232 and SRR2014233) replicates from the study are reported here. ALFA enables, with a single command, to quickly confirm that mitochondrial rRNA contamination is more important in the heart samples than in the lung samples (t-test significant at a 1% level with a p-value of 3 × 10^–3). Moreover, an intergenic contamination, not revealed in the original work, can also be noticed on Lung Adult-Rep2 with no additional work.