Fig. 3From: Development of PCR markers specific to Dasypyrum villosum genome based on transcriptome data and their application in breeding Triticum aestivum-D. villosum#4 alien chromosome linesAmplification of candidate primers specific to D. villosum#4 targeted chromosomes designed by D. villosum#4 RNA-seq unigenes. M: marker 5000; 1: CS; 2: DA5V#3; 3: D. villosum#4 accession No. 1026. a: amplified by marker 5V-13; b: amplified by marker 5V-40F3R3; c: amplified by marker 5V-14; d: amplified by marker 5V-15Back to article page