Fig. 6

Genome mining of Fengycin, Bacitracin and Paralichenicidin-like gene clusters. (a) Genetic organization of gene clusters of Fengycin. Same genes are shown in the same color and linked by dotted lines. (b) Genetic organization of gene clusters of Bacitracin. Percentage amino-acid identities of each peptide with Bacitracin operon in B. paralicheniformis MDJK30 are shown. Insertion sequence, biosynthetic genes, transport genes, regulatory genes and other genes are shown in black, red, blue, green and gray, respectively. A domain substrates in biosynthetic genes are predicted by antiSMASH [16]. (c) Genetic organization of gene clusters of Paralichenicidin and Lichenicidin. Region in inset box compares two amino acid sequences of the core peptide LanA1 and LanA2 of the structural components of Paralichenicidin and Lichenicidin. (d) Genetic organization of related gene clusters of Paralichenicidin. Percentage amino acid identities of each peptide with Paralichenicidin peptide are shown. (e) Sequence alignment of LanA1 and LanA2. Completely conserved and highly conserved residues are indicated by asterisks and points, respectively. Completely conserved residues in core peptides are shown in gray boxes. Ser, Thr and Cys residues in the core peptide are shown in purple, green and blue, respectively. Leader cleavage site is predicted by antiSMASH [16] and shown in yellow