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Table 1 Mutations associated with resistance to amoxicillin, enrofloxacin, kanamycin, or tetracycline in wild-type E. coli

From: Effects of a previously selected antibiotic resistance on mutations acquired during development of a second resistance in Escherichia coli

Exposure to amoxicillin
  WT WT
Gene product Gene Mutation 40 μg/ml 1280 μg/ml 1280 μg/ml
 B-lactamase ampC G23 T (promoter) 1 1 1
C-11 T (promoter) 1 1 1
 Transcriptional regulator ompR E88A   1 1
 Citrate lyase citF G432A   1 0.97
 Sigma 70 rpoD D445V   0.96 1
 UDP-D-galactose waaB Del C3803246 (FS297)    1
Exposure to enrofloxacin
   WT WT
Gene product Gene Mutation   1024 μg/ml 1024 μg/ml
 DNA gyrase subunit A gyrA D87G   1  
S83 L   1 1
D87N    1
 DNA gyrase subunit B gyrB S463F    1
 DNA topoisomerase IV subunit A parC S80R   1 1
 DNA topoisomerase IV subunit B parE Ins Q458   0.94 1
 Cryptic proline/histidine transporter proY A235S   1 1
 Transcriptional regulator acrR Del 485,885–11 (FS42)   1 1
 Transcriptional regulator soxR T133S, del 4,277,899–903 (FS144)   1 1
 S10 subunit of 30S rpsJ 5′ UTR (G3453306 T)   1 1
 Putative transport protein/Putative inner membrane protein ybjL/ybjM* 5′ UTR (A889923G)   1 1
 Glutamate synthase subunit gltB 5′ UTR (C3354487T)   1 1
 Putative RNA binding protein yciO N64Y   1 1
 Mismatch repair protein mutL W390 L    0.12
Exposure to kanamycin
  WT WT
Gene product Gene Mutation 32 μg/mL 1024 μg/mL 1024 μg/mL
 Peptide antibiotic transporter sbmA Del 397,306–27 (FS 222)   1 1
 ATP binding protein of putrescine ABC exporter sapF L181Q   1 1
 Sensor protein of Cpx TCS cpxA Q242L   1 1
 DNA gyrase subunit B gyrB S464Y   1 1
 Phosphorelay protein npr L23R   1 1
 Elongation factor G fusA Q242L   1  
A608V 0.19   
F05 L 0.74   1
 β-D-glucuronidase uidA 5′ UTR (T1696220C)    1
Exposure to tetracycline
  WT  
Gene product Gene Mutation 16 μg/mL 64 μg/mL  
 Multidrug efflux pump RND permease acrB I45L 1 1  
 Transcriptional regulator acrR P85Q 1 1  
 Outer membrane porin F ompF T71S 0.65 0.04  
Ins 7 nt after G986771 (FS71)   0.60  
 Outer membrane lipoprotein mlaA Del N41/F42   0.9  
 RNA polymerase subunit β’ rpoC G367C 0.34   
  1. Numbers shown indicate frequency of mutation in population. Del = deletion, Ins = insertion, underlined letters and numbers indicate nucleotides and their genomic position, * indicates that mutation could affect either gene. For amoxicillin, enrofloxacin, and kanamycin, two independent strains were sequenced