Table 1 Mutations associated with resistance to amoxicillin, enrofloxacin, kanamycin, or tetracycline in wild-type E. coli

WT

Gene product

Gene

Mutation

40 μg/ml

1280 μg/ml

1280 μg/ml

 B-lactamase

ampC

G23 T (promoter)

1

1

1

C-11 T (promoter)

1

1

1

 Transcriptional regulator

ompR

E88A

1

1

 Citrate lyase

citF

G432A

1

0.97

 Sigma 70

rpoD

D445V

0.96

1

 UDP-D-galactose

waaB

Del C3803246 (FS297)

1

WT

WT

Gene product

Gene

Mutation

1024 μg/ml

1024 μg/ml

 DNA gyrase subunit A

gyrA

D87G

1

S83 L

1

1

D87N

1

 DNA gyrase subunit B

gyrB

S463F

1

 DNA topoisomerase IV subunit A

parC

S80R

1

1

 DNA topoisomerase IV subunit B

parE

Ins Q458

0.94

1

 Cryptic proline/histidine transporter

proY

A235S

1

1

 Transcriptional regulator

acrR

Del 485,885–11 (FS42)

1

1

 Transcriptional regulator

soxR

T133S, del 4,277,899–903 (FS144)

1

1

 S10 subunit of 30S

rpsJ

5′ UTR (G3453306 T)

1

1

 Putative transport protein/Putative inner membrane protein

ybjL/ybjM*

5′ UTR (A889923G)

1

1

 Glutamate synthase subunit

gltB

5′ UTR (C3354487T)

1

1

 Putative RNA binding protein

yciO

N64Y

1

1

 Mismatch repair protein

mutL

W390 L

0.12

WT

Gene product

Gene

Mutation

32 μg/mL

1024 μg/mL

1024 μg/mL

 Peptide antibiotic transporter

sbmA

Del 397,306–27 (FS 222)

1

1

 ATP binding protein of putrescine ABC exporter

sapF

L181Q

1

1

 Sensor protein of Cpx TCS

cpxA

Q242L

1

1

 DNA gyrase subunit B

gyrB

S464Y

1

1

 Phosphorelay protein

npr

L23R

1

1

 Elongation factor G

fusA

Q242L

1

A608V

0.19

F05 L

0.74

1

 β-D-glucuronidase

uidA

5′ UTR (T1696220C)

1

Gene product

Gene

Mutation

16 μg/mL

64 μg/mL

 Multidrug efflux pump RND permease

acrB

I45L

1

1

 Transcriptional regulator

acrR

P85Q

1

1

 Outer membrane porin F

ompF

T71S

0.65

0.04

Ins 7 nt after G986771 (FS71)

0.60

 Outer membrane lipoprotein

mlaA

Del N41/F42

0.9

 RNA polymerase subunit β’

rpoC

G367C

0.34