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Table 4 Mutations associated with resistance to kanamycin in strains with a previously acquired resistance to amoxicillin, enrofloxacin, or tetracycline

From: Effects of a previously selected antibiotic resistance on mutations acquired during development of a second resistance in Escherichia coli

Gene product

Gene

Mutation

Pop. frequency

Strains

Multidrug efflux pump RND permease

acrB

V139F

0.73–1

E2/3

Inner membrane magnesium ion transporter

corA

Del V264-F266

0.93

T1/4

Sensor protein of Cpx two-component system

cpxA

Q242L

1

E2/3

Cytochrome bo3 ubiquinol oxidase subunit 1

cyoB

G662C

0.86–0.93

E2/3

Del G448658 (FS661)

0.13

E1/3

Start1L

0.12

E1/3

Cytochrome bo3 ubiquinol oxidase subunit 3

cyoC

Start1I

0.86–0.93

E2/3

Del C448658 (FS1)

0.13

E1/3

N661Stop

0.12

E1/3

Formate dehydrogenase O subunit α

fdoG

V226 L

0.36–0.58

A2/3

Elongation factor G

fusA

T393I

1

A1/3

F605 L

1

E3/3, T3/4

P610L

0.92–0.97

A2/3

P610T

0.96

T1/4

sensory histidine kinase

kdpD

Q728P

0.36–0.61

A1/3, T1/4

tRNA-Leu

leuP

V16 V

0.67

A1/3

Outer membrane lipoprotein

mlaA

Q728P

0.88

T1/4

Del F42-N43

0.92

T1/4

2-O-α-mannosyl-D-glycerate specific PTS permease

mngA

F431 L

0.28–0.59

A2/3

Mismatch repair protein

mutL

W390 L

0.11–0.13

A1/4, T2/4

Iron-sulfur cluster carrier protein

nfuA

Ins 8 nt after G3546085 (FS154)

0.23

A1/3

NADH:quinone oxidoreductase subunit CD

nuoC

Del R471-L474

0.93

E1/3

NADH:quinone oxidoreductase subunit G

nuoG

Ins C after A2399396 (FS257)

0.4

A1/3

Murein/oligopeptide ABC transporter subunit

oppF

L888Stop

0.74–1

E2/3

Ins SIQ after L187

0.14

E1/3

Aminopeptidase N

pepN

V809E

0.57

A1/3

RNA polymerase subunit β’

rpoC

G367C

0.16

E1/3

ATP binding protein of putrescine ABC exporter

sapF

L181Q

1

T2/4

Peptide antibiotic transporter

sbmA

Ins G after T393897 (FS88)

0.99

E1/3

W98Stop

0.44

A1/3

Y162Stop

0.11

T1/4

W179Stop

0.53–0.60

T2/4

S250Stop

0.44

A1/3

L369 L

0.14

T2/4

I370L

0.14

T2/4

Uncharacterized protein

ygaQ

Q417K

1

E2/3

Uncharacterized protein

ypjC

5′ UTR (T2785462C)

1

E2/3

Putative transporter

yqeG

P282P

0.22

E1/3

Putative fimbrial usher protein

yraJ

Q614L

0.18

T1/4

H615H

0.19

T1/4

  1. Del = deletion, Ins = insertion, underlined letters and numbers indicate nucleotides and their genomic position. Population frequency indicates the mutational frequency in the sequenced population. Strains indicates the prevalence of a mutation within different replicates. A: population with previously acquired resistance to amoxicillin, E: population with previously acquired resistance to enrofloxacin, T: population with previously acquired resistance to tetracycline. X/Y: X indicates number of replicates with particular mutation, Y indicates total number of replicates that were sequenced. Mutations associated with resistance in a wild-type background are shown in bold. Mutations associated with the previous resistance have been omitted from the table
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BMC Genomics

ISSN: 1471-2164

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