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Table 5 Mutations associated with resistance to tetracycline in strains with a previously acquired resistance to amoxicillin, enrofloxacin, or kanamycin

From: Effects of a previously selected antibiotic resistance on mutations acquired during development of a second resistance in Escherichia coli

Gene product Gene Mutation Pop. frequency Strains
Multidrug efflux pump membrane fusion lipoprotein/multidrug efflux pump RND permease acrA/acrB* 5′ UTR (Del 485,695–711) 0.77 A1/4
Multidrug efflux pump RND permease acrB V139F 1 A1/4, E4/4
S665A 1 A1/4
Transcriptional regulator acrR I45L 0.97 K1/1
P85Q 1 A3/4, K1/4
E130K 1 K1/1
R442Q 1 K1/1
Crotonobetainyl-CoA reductase caiA C154A, F155P, I156T, T157P, S158R, S159 T, A160S, Y161W, Del T162-R380 1 E1/4
Endochitinase chiA W702R 1 K1/1
Membrane associated sensor kinase envZ P247S 0.24–0.95 A1/4, K1/1
3-ketoacyl-CoA thiolase fadI L246 L 1 E2/4
Peptidoglycan DD-transpeptidase ftsI A513S 1 K1/1
DNA-binding transcriptional repressor marR Ins C after A1619472 (FS118) 1 A1/4
ABC transporter family protein mdlB A16A 1 A2/4
mlaA Del F42-N43 0.93 K1/1
Murein tripeptide ABC transporter periplasmic binding protein mppA F244F 1 K1/1
Mismatch repair protein mutL W390 L 0.1 E1/4
Outer membrane porin F ompF Ins 7 nt after G986771 (FS71) 0.04 E1/4
Truncated RNase PH rph Del R676 1 E1/4
RNA polymerase subunit β’ rpoC Del T208-K213 1 E1/4
DNA-binding transcriptional dual regulator soxR T145S, G146 T, A147L, R148A, L149G, L150R, Del E151-N155 1 E1/4
DNA-binding transcriptional dual regulator soxS S2F 0.98–1 E3/4
Bifunctional (p) ppGpp synthase/hydrolase spoT Ins C after T3815879 (FS223) 1 E1/4
DNA-binding transcriptional activator ybaO T33P 1 E1/4
  1. Numbers shown indicate frequency of mutation in population. Del = deletion, Ins = insertion, underlined letters and numbers indicate nucleotides and their genomic position. Population frequency indicates the mutational frequency in the sequenced population. Strains indicates the prevalence of a mutation within different replicates. A: population with previously acquired resistance to amoxicillin, E: population with previously acquired resistance to enrofloxacin, K: population with previously acquired resistance to kanamycin. X/Y: X indicates number of replicates with particular mutation, Y indicates total number of replicates that were sequenced. Mutations associated with resistance in a wild-type background are shown in bold. Mutations associated with the previous resistance have been omitted from the table