Fig. 2

Comparison of TSSs between translatome and transcriptome. a A typical example of TSS distribution disparity. The two TCs are located in at the core promoter region of the gene “EIF3D”. The red color stands for the 5′ end distribution in translatome and the green stands for the distribution in transcriptome (the yellow is the common part of the two distributions). b The distance between representative TSSs of translatome and transcriptome. The distance is calculated with the genomic coordinates of representative TSSs on the human genome. c Comparison of the nucleotide frequency at representative TSSs in translatome and transcriptome. d Usage landscape of HEK293-derived representative TSSs in 180 human samples. All 3248 representative TSSs are from HEK293 translatome and only those from chromosome 1 are displayed here for better visualization. In the heatmap, “1” (marked with red) means the representative TSS on the right side is also used as representative TSS in the sample on the top. The histogram on top of sample names shows the number of representative TSSs used by each sample. The name of each representative TSS contains the information of chromosome, strand and genomic position. For the full list of all 3248 representative TSSs, please refer to Additional file 5: Table S3