Transcriptional profiling and pathway analysis reveal differences in pituitary gland function, morphology, and vascularization in chickens genetically selected for high or low body weight

Table 2 Summary of microarray analysis

Category	Number
Spots printed on array	19,200
Spots with cDNA probes	17,834
Unique genes represented	14,053
^acDNA probes within trimmed dataset	10,437
^bSignificant cDNA probes	2310
Line-by-age interaction	353
Main effect of line	1100
Main effect of age	1058
^cDifferentially expressed genes	291
Line-by-age Interaction	110
Main effect of line	181
Main effect of age	72
^dGenes annotated with GeneBase	260

^acDNA probes remaining after quality control check for removal of any that were undetectable, below background, malformed, had saturated pixel intensity, or were not present on at least half of the microarray slides
^bcDNA probes with detectable background-corrected signal on at least 16 microarray slides and significantly different at P ≤ 0.05
^ccDNA probes with detectable background-corrected signal on at least 16 microarray slides, P ≤ 0.05 for line-by-age interaction or main effect of line, and a difference between groups of 1.6-fold or greater (0.68-fold on a log₂-scale)
^dNumber of 291 differentially expressed genes successfully annotated with protein identifiers using the GeneBase tool at http://cogburn.dbi.udel.edu/

ISSN: 1471-2164