Table 2 The bacterial strains and plasmids used in this work
Strains and plasmids | Description | Reference |
|---|---|---|
Strains | ||
 Escherichia coli DH5α | supE44ΔlacU169 (Φ80 lacZΔM15) hdsR17 recA1 endA1 gyrA96 thi-1 relA1 | Lab stock |
 Bacillus pumilus BA06 | The wild-type strain | Lab stock |
 B. pumilus BA06-∆scoC | ∆scoC; Cm | This work |
 B. pumilus (∆scoC/scoC+) | scoC+; Kan | This work |
Plasmids | ||
 pMD-18 T | T-vector; Amp; high copies | TaKaRa Co. |
 pHCMC02 | Cm; high copies | [45] |
 pUCETs | Shuttling vector (E. coli and Bacillus); temperature-sensitive ori; Erm; low copies | [46] |
 pSU03-Ap | expression vector (Bacillus); high copies | [47] |
 p18T-scoC::cm | scoC::Cm; Amp; high copies | This work |
 pUCETs:scoC::cm | scoC::Cm; Erm; low copies | This work |
 pSU03-scoC | scoC; promoter of GAPD; Kan; high copies | This work |