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Table 2 The bacterial strains and plasmids used in this work

From: Disruption of the pleiotropic gene scoC causes transcriptomic and phenotypical changes in Bacillus pumilus BA06

Strains and plasmids Description Reference
Strains
Escherichia coli DH5α supE44ΔlacU169 (Φ80 lacZΔM15) hdsR17 recA1 endA1 gyrA96 thi-1 relA1 Lab stock
Bacillus pumilus BA06 The wild-type strain Lab stock
B. pumilus BA06-∆scoC scoC; Cm This work
B. pumilus (∆scoC/scoC+) scoC+; Kan This work
Plasmids
 pMD-18 T T-vector; Amp; high copies TaKaRa Co.
 pHCMC02 Cm; high copies [45]
 pUCETs Shuttling vector (E. coli and Bacillus); temperature-sensitive ori; Erm; low copies [46]
 pSU03-Ap expression vector (Bacillus); high copies [47]
 p18T-scoC::cm scoC::Cm; Amp; high copies This work
 pUCETs:scoC::cm scoC::Cm; Erm; low copies This work
 pSU03-scoC scoC; promoter of GAPD; Kan; high copies This work