Fig. 3

Global profile of H3K9me3 and H3K4me3. (a) The heatmaps display H3K9me3 enrichment for a subset of genes in WT and ∆kmt2/∆set-1. The blue arrow highlights genes enriched for H3K9me3 in the gene body in both WT and ∆kmt2/∆set-1, while the blue arrow indicates transcripts that had elevated H3K9me3 in ∆kmt2/∆set-1. (b) Similar to panel (a), except we examine H3K4me3 in WT and ∆kmt1/∆dim-5. Three independent clusters show the spatial distribution of H3K4me3 for transcript identified in Fig. 1a and included 2 kb upstream of TSS and downstream of TES. Clusters not shown for H3K9me3 and H3K4me3 did not have a ChIP-seq peak associated with them. (c & d) The MAplots show quantitative difference in H3K9me3 peak densities between WT and ∆kmt2/∆set-1 in (c) DD and (d) LP30. (e-f) Same as in (c) & (d) except differences in H3K4me3 peak densities were between WT and ∆kmt1/∆dim-5. The red spots on the MA plots indicate a log fold change (p < 0.05)