Fig. 6

H3K4me3 and H3K9me3 can be co-dependent. (a). Venn diagram show presumptive K4/K9 bivalent domains in WT that contain both H3K4me3 and H3K9me3 and the extent of overlap in DD and LP30. (b) The gene level plot displays H3K9me3 ChIP-seq (DD, Blue and LP30, Navy) in WT and ∆kmt2/∆set-1 and H3K4me3 ChIP-seq (DD, Grey and LP30, black) in WT and ∆kmt1/∆dim-5 at divergently transcribed NCU09968 and NCU09969. Relative expression of transcripts originating from either the plus (Green) or minus (Orange) strands is shown for the 3 strains. NCU09969 also has an antisense transcript(s) identified by SringTie (not drawn). Expression level of (c) NCU09968 and (d) NCU09969 are shown as bar plots in WT, kmt2/∆set-1 and kmt1/∆dim-5 for DD (grey) and LP30 (black). (e) Reciprocal sequential ChIP (re-ChIP) in WT from 3 independent biological replicates. The first antibody (1^o) and second antibody (2^o) used in the re-ChIP are shown above the bar plot and the amount recovered from the re-ChIP was determined by determine by qPCR and represented as a percentage of the input (Relative level, % Total). The red asterisk on the gene-level plots show the location of the oligo used for re-ChIP (f) For each re-ChIP experiment, we removed one tenth of the primary ChIP and measured the amount recovered as a control using the antibodies listed above the graph