Fig. 7From: Gene expression profiling analysis to investigate the role of remote ischemic postconditioning in ischemia-reperfusion injury in ratsqPCR analysis data targeting several genes. To further verify the differentially expressed mRNAs, a qPCR assay was performed. The 2-ΔΔCT method was applied to calculate the relative transcript levels. The relative expression of Gapdh was utilized to normalize the expression of tested genes, including Plcb4, Pdgfra, Ccr1l1, Stat1, Jun, Hba2, LOC501110, Stfa3, Cflar, Lifr, and Gstm5. Independent sample Student’s t-test was also performed for the statistical analysis. Significant difference was indicated: *, P < 0.05; **, P < 0.01Back to article page