Table 2 Genes and sequences of primers used for validation by droplet digital PCR (ddPCR)
Locus | Gene name | Product length (nt) | Primer F (5′- > 3′ on plus strand) | Primer R (5′- > 3′ on minus strand) | Tm (Celsius) | G + C (%) |
|---|---|---|---|---|---|---|
6 Genes: Six genes selected because they are differentially expressed in three temporary evaluation points (1 h, 48 h and 96 h) in Bacillus and Pseudomonas treatments | ||||||
 LOC103994690 | L-ascorbate oxidase homolog | 139 | TCTCACCACCAGCCAATTCC | ATGCAGCAGAGACACAACGA | 60 | 50–55 |
 LOC103978608 | Wound-induced protein 1-like | 70 | CTAACATGGTCCTCGGCTCG | AGTACACTCAGATCGGCCCT | 60 | 55–60 |
 LOC103988136 | Cytochrome C | 94 | AACATGTTGCCCTGCTTGTG | TCAGGCAAGAGCCCATGAAA | 60 | 50 |
 LOC103998260 | Cytochrome P450 714B3-like | 136 | ACTCTTCTCACAAGGCCGAC | TCAACTCTGCCCATGACACA | 60 | 50–55 |
 LOC103983858 | Ubiquinol oxidase 2, mitochondrial-like | 115 | GCGGCTTGTTTGGTGTTTCT | AGGAACGTACCCAATGCTGG | 60 | 50–55 |
 LOC103999297 | Palmitoyl-acyl carrier protein thioesterase, chloroplastic | 140 | TTAAGGCGCAAGCACAAACC | CAGAAAGCTCCAGTCAGGCA | 60 | 50–55 |
4 Genes: Four genes selected because they are differentially expressed in three temporary evaluation points (1 h, 48 h, 96 h) under Pseudomonas treatment | ||||||
 LOC103971944 | Caffeic acid 3-O-methyltransferase | 144 | GGTGGAGGAGACGCCATTTT | CGGAATCACGGGAAGCAAGT | 60 | 55 |
 LOC103975393 | Heat shock cognate 70KDa protein | 143 | ACGAGGGTGAGAGGACAAGA | GGCGGACACGTTTATGATGC | 60 | 55 |
 LOC103975709 | Small heat shock protein | 100 | GCCTCAGCGACTACCTTACC | ATGCTACTGTCGAAGGCTGG | 60 | 55–60 |
 LOC103978845 | Truncated transcription factor CAULIFLOWER | 108 | GCTCTACGAGTACTCCACCG | TCCTGGGATTCAGTGTCTGC | 60 | 55–60 |
12 Genes: Twelve genes selected because they are differentially expressed in three temporary evaluation points (1 h, 48 h, 96 h) under Bacillus treatment | ||||||
 LOC103998403 | Heat shock cognate 70KDa protein 8 | 71 | ATCGGCCTAGGTGAAGATGG | TGTCGTCACCGGTTGTTTCA | 60 | 50–55 |
 LOC103986775 | U-Box domain-containing protein 21-like | 74 | CTCATGTCGGCAACTCTCGT | CGTCCAAATCCCCAGACCTC | 60 | 55–60 |
 LOC103997576 | Horcolin | 78 | TAACGCTGGGGACATCATCG | CCACCGTAGTGTTGGGTCTC | 60 | 55–60 |
 LOC103991351 | Leucine-rich repeat extensin-like protein 4 | 81 | CACCTGAGTGCATGGGGATT | CCCTAGAGCAAGCTACAGCC | 60 | 55–60 |
 LOC103971548 | Cyclic dof factor 3-like | 86 | GCACAGGCTTCTGATCTGGT | GCCTTGACAAACCTTGCCTG | 60 | 55 |
 LOC103976748 | CASP-like protein 4D1 | 89 | AATGGCCTCTTCGACCAAGG | TGACGACGAGGGAAACCAAG | 60 | 55 |
 LOC103987539 | U-Box domain-containing protein 21-like | 89 | CCACGCGATACGGAAGATGA | TCACCGGAATCTTAGGCGTG | 60 | 55 |
 LOC103990849 | Serine/threonine-protein kinase | 115 | AACTGATCGGGTACTGCTGC | GACCACGGCAACGAAGAAAC | 60 | 55 |
 LOC103972371 | Cycle dof factor 3-like | 131 | CGCACAGCTATTGCACGAAA | AGCTCCCAAATCACATGGCA | 60 | 50 |
 LOC103989093 | Abscisic stress-ripening protein 2 | 139 | TCGGAGACTGCCTACTCTGG | CGCCCATCTTTCCGAGATGT | 60 | 55–60 |
 LOC103985511 | Trascription factor Bhlh 130 | 143 | TCTCGTCGAGGCAAAACTCC | GGAATCGTCCCAAGAGCCAA | 60 | 55 |
 LOC103977424 | Pectinesterase-like | 150 | GGGAACCACCGTTACGACAT | GCTGTTGGCAATGAGTGAGC | 60 | 55 |