Cytogenetic and molecular genotyping in the allotetraploid Festuca pratensis × Lolium perenne hybrids

BMC Genomics

Table 1 Comparison of nine selected cytogenetic trait distributions in F₂ generation of F. pratensis × L. perenne hybrid

Trait	Value of the trait for F₁ parental plants			P-value for equality of distributions in F₂ generation^a	Mean value in F₂ generation		P-value for equality of mean values in F₂ generation^b	P-value for mean within generation being equal to mid-parent value^c
Trait	F₁ 6-2A	F₁ 6-7A	F₁ 6-3B	P-value for equality of distributions in F₂ generation^a	Combination no. 1	Combination no. 2	P-value for equality of mean values in F₂ generation^b	Combination no. 1	Combination no. 2
Number of 35S rDNA in Lp genome	7	6	7	0.057	6.42	6.98	0.005	0.509	0.884
Number of 35S rDNA in Fp genome	2	2	2	0.656	1.89	1.96	0.465	0.096	0.486
Number of 5S rDNA in Lp genome	2	2	2	1.000	1.96	1.98	0.562	0.160	0.323
Number of 5S rDNA in Fp genome	2	2	2	1.000	2.04	2.04	1.000	0.160	0.160
Number of 1, 2, 7L chromosomes	5	4	5	0.075	4.47	5.00	0.007	0.769	1.000
Number of 3L chromosomes	2	2	2	1.000	1.96	1.98	0.562	0.160	0.323
Number of 2F chromosomes	2	2	2	0.675	1.89	1.96	0.465	0.096	0.486
Number of 3F chromosomes	2	2	2	1.000	2.04	2.04	1.000	0.160	0.160
Number of chromosomes M-	17	18	16	0.024	17.53	17.00	0.009	0.785	0.003

(Lp) L. perenne; (Fp) F. pratensis; (M+) rDNA-bearing chromosome; (M-) non-rDNA-bearing chromosome; (m+) chromosome arm with rDNA sequence; (m-) chromosome arm without rDNA sequence; bold P-values for comparisons considered as statistically significant
^aP-value for the χ² test of equal distributions of the trait in F₂ combinations no.1 and 2 (based on permutations)
^bP-value for the t-test of equality of mean values for the trait in F₂ combinations no. 1 and 2
^cP-value for the t-test of equality of mean value in F₂ generation and mean of parents

ISSN: 1471-2164