Cytogenetic and molecular genotyping in the allotetraploid Festuca pratensis × Lolium perenne hybrids

BMC Genomics

Table 2 Comparison of nine selected cytogenetic trait distributions in F₂ and F₃ generations of F. pratensis × L. perenne hybrid

Trait	Value of the trait for F₂ parental plants				P-value for equality of distributions in F₂ and F₃ plants	Mean value in generation		P-value for equality of mean values in F₂ and F₃ plants
Trait	2A-15	2A-5	2A-27	2A-12	P-value for equality of distributions in F₂ and F₃ plants	F₂	F₃	P-value for equality of mean values in F₂ and F₃ plants
Number of 35S rDNA in Lp genome	6	8	7	7	0.002	6.42	7.32	< 0.001
Number of 35S rDNA in Fp genome	2	0	2	2	< 0.001	1.89	1.46	< 0.001
Number of 5S rDNA in Lp genome	2	2	2	2	0.239	1.96	1.98	0.718
Number of 5S rDNA in Fp genome	2	2	2	2	0.123	2.04	1.98	0.328
Number of 1, 2, 7L chromosomes	4	6	5	5	< 0.001	4.47	5.32	< 0.001
Number of 3L chromosomes	2	2	2	2	0.245	1.96	1.98	0.718
Number of 2F chromosomes	2	0	2	2	< 0.001	1.89	1.48	< 0.001
Number of 3F chromosomes	2	2	2	2	0.105	2.04	1.98	0.328
Number of chromosomes M-	18	17	17	17	0.040	17.53	17.11	0.027

(Lp) L. perenne; (Fp) F. pratensis; (M+) rDNA-bearing chromosome; (M-) non-rDNA-bearing chromosome; (m+) chromosome arm with rDNA sequence; (m-) chromosome arm without rDNA sequence; bold P-values for comparisons considered as statistically significant

ISSN: 1471-2164