Fig. 4

Commonly up-regulated DEUs from s-H vs c-H, s-F vs c-F and s-A⁺ vs c-A⁺. a Venn diagram of up- or down-regulated DEUs from s-A⁺ vs c-A⁺ (green circle), s-F vs c-F (blue circle) and s-H vs c-H (red circle). b The qPCR analyses of eight genes (sptb, urod, cpox, fech, blvrb, hba, mfrn1 and fam210b) related to circulatory system in head kidney of gibel carp clone A⁺, F and H. eef1a1l1 was used as the normalizer. Gene expression levels are relative to that of clone A⁺ control individuals. Each bar represents mean ± standard deviation (SD) (n = 3). Asterisks indicate the significant differences between control and survivor (*: p < 0.05 and **: p < 0.05). c Heatmap of commonly up-regulated DEUs among s-H vs c-H, s-F vs c-F and s-A⁺ vs c-A⁺. Hierarchical clustering is calculated by log₂ fold change values and the main clusters were lined out on the right