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Table 2 Summary statistics of S. argus transcriptome. *FPKM: fragments per kilobase per transcript per million mapped reads

From: Transcriptional analysis of renal dopamine-mediated Na+ homeostasis response to environmental salinity stress in Scatophagus argus

Sequencing

Number

Before trimming

 Raw reads (pair-end)

1,023,047,770

After trimming

 Clean reads (pair-end)

575,312,926

 Total nucleotide (bp)

7.22 × 1010

After assembly

 

De Novo assembled

Filtered (FPKM* ≥ 1)

 Transcripts

186,397

43,933

 N50 length (bp)

2521

3118

 N90 length (bp)

462

978

 Reads Mapped

1.94 × 108

1.26 × 108

 GC content (%)

43.20

45.02

 Min length (bp)

224

 Max length (bp)

23,112

 Average length (bp)

1272

2022

Annotation

 NCBI-NR

14,259

 SWISS-PROT

8360

 GO

12,247

 KEGG

11,354

 KOG

3168