Concurrent and long-term associations between the endometrial microbiota and endometrial transcriptome in postpartum dairy cows

Table 4 Predicted upstream regulators of endometrial genes differentially expressed at week 9 (day 13 of the estrous cycle) in response to WK1_PC2

Upstream regulator	Z-score^a	P-value	Differentially expressed genes (week 9)^c
Bacterial products
Mma_DMAG^b	2.45	5.67E-08	CCL20, CXCL2, NFKBIZ, SDC4, SOD2, TNIP3
Enterotoxin B	2.41	2.88E-08	CSF3, CTLA4, CXCL13, CXCL2, IL12B, IL17A
Lipopolysaccharide	3.63	9.88E-10	CCL20, CHI3L1, CSF3, CXCL13, CXCL2, IFNW1, IL12B, IL17A, IL22, IL36A, LAMB1, LCN2, LYN, NFKBIZ, PC, PFKFB3, RAB20, SDC4, SOD2, TNIP3
Peptidoglycan	2.39	2.65E-07	CCL20, CSF3, CXCL2, IL12B, IL17A, SOD2
Interleukins
IL1	2.28	1.40E-06	CCL20, CHI3L1, CSF3, IL17A, IL22, LAMB1, SDC4, SOD2
IL1A	2.56	6.29E-08	ALDH1A3, CCL20, CXCL2, IL12B, IL17A, LCN2, NFKBIZ, SOD2
IL1B	3.39	1.30E-09	CCL20, CHI3L1, CSF3, CXCL13, CXCL2, GDF15, IL12B, IL17A, IL22, IL36A, LCN2, NFKBIZ, SDC4, SLC22A4, SOD2
IL6	2.75	5.56E-05	CCL20, CLEC10A, CXCL13, CXCL2, IL12B, IL17A, IL22, LCN2, SOD2
IL10RA	−2.00	9.07E-04	CTLA4, IL12B, IL17A, IL18BP, LCN2
IL17A	2.18	5.87E-08	CCL20, CSF3, CXCL13, CXCL2, IL17A, IL22, LCN2, NFKBIZ
Tumor necrosis factor	3.62	2.72E-12	AATK, ALDH1A3, CCL20, CHI3L1, CHI3L2, CSF3, CTLA4, CXCL13, CXCL2, GDF15, IL12B, IL17A, IL18BP, IL22, IL36A, ITGA10, LCN2, LYN, NFKBIZ, PC, SDC4, SLC22A4, SOD2
Toll-like receptors
TLR3	2.91	8.70E-09	CCL20, CSF3, CXCL2, IFNW1, IL12B, IL36A, LCN2, NFKBIZ, PFKFB3
TLR4	2.88	1.04E-09	CCL20, CSF3, CXCL2, DUOX2, IFNW1, IL12B, IL17A, IL18BP, LCN2, NFKBIZ, SOD2
TLR5	2.18	2.81E-08	CCL20, CSF3, CXCL2, IL12B, IL17A
TLR7	2.39	1.52E-06	CCL20, CXCL13, CXCL2, IFNW1, IL12B, SOD2
TLR9	2.57	1.07E-06	CSF3, IFNW1, IL12B, IL36A, LCN2, NFKBIZ, PFKFB3

^apositive z-score = increased activation; negative z-score = decreased activation
^b5-O-mycolyl-beta-araf-(1 → 2)-5-O-mycolyl-alpha-araf-(1 → 1′)-glycerol
^cSee Additional file 1: Table S10 for gene description

ISSN: 1471-2164