Application of high-throughput amplicon sequencing-based SSR genotyping in genetic background screening

BMC Genomics

Table 2 Genotypes of the differential SSRs between D62B and DXB included in the National Agricultural Standard of China

Amplicon	D62B			DXB			Name in national standard
Amplicon	Genotype	Coverage	Stutter ratio	Genotype	Coverage	Stutter ratio	Name in national standard
AMPL1563384	TC10	47	0.13	TC9	19	0.32	RM561
AMPL1563300	TCA16	45	0.16	TCA15	20	0.15	RM19
AMPL1563868	CA6	88	0.40	CA5	77	0.31	RM316
AMPL1563825	AAG8	265	0.08	AAG11	104	0.13	RM339
AMPL1563618	GCA8	247	0.15	GCA9	179	0.40	RM598
AMPL1563371	ATG18	198	0.31	ATG15	189	0.20	RM424
AMPL1563368	AAT6	481	0.07	AAT4_AAT10	284	0.08	RM71
AMPL1563350	GAA15	192	0.11	GAA8	694	0.06	RM423

The coverage represents the number of reads that support the genotype. The stutter ratio represents the ratio of the numbers of reads for the minor and major genotypes

ISSN: 1471-2164