Table 4 Primers used for qRT-PCR validation of Pfu. mantle edge tissues transcriptome data
Primer name | Gene name | Sequences (5′-3′) |
|---|---|---|
18S-F | 18S | CGTTTCAACAAGACGCCAGTAG |
18S-R | ACGAAAAAAAGGTTTGAGAGACG | |
c50039-RT-F | Nacre protein | TTCATCGCTACCATTACAACGG |
c50039-RT-R | TCGTCGGAAGATTACAGCATTC | |
c62280-RT-F | Nacre protein, N16.3 | TCTGTAGATACGCTTGGTCTCC |
c62280-RT-R | TTCCGTTACCGTTGTCATCATC | |
c70362-RT-F | Nacre protein | CTTGGACAGGCACAGACTCA |
c70362-RT-R | CTGAATGGTGTTCTTCGGCAAT | |
c68187-RT-F | Shell matrix protein | CCAGTGCTTCCAATACCAAGG |
c68187-RT-R | TCCAGTGCGGTTGTATGTGTA | |
c61883-RT-F | Shell protein 1 | ATTCAGTTCCGTACCTGTTCCT |
c61883-RT-R | ACATTGTTGGCGTCTAGTCTCT | |
c56018-RT-F | Shell protein 12 | TAGGAGGAGCACAGCACTTG |
c56018-RT-R | ACGAGGTTGACTGTAGGTGTTA | |
c55873-RT-F | Mantle gene 12 | CGTTCATAGACTGCCAACATG |
c55873-RT-R | TAAGACTGACGCCGACTGT | |
c77975-RT-F | Mantle gene 4 | TCCGAGAGGTAATGTTGTCTAC |
c77975-RT-R | CCGTTGTACTCAGTGGAAGAA | |
c45445-RT-F | Mantle gene 6 | CCTTTACGCATTGGTTGATACG |
c45445-RT-R | TGAAGAGTTGGCTGAAGTGTTC | |
c59543-RT-F | Mantle gene 1 | AAGGATGGTGGAGAAGGACAA |
c59543-RT-R | TCGTAAGAAGCTAGTTCACAGG | |
c72378-RT-F | Calmodulin (CaM) | ATTGGGACAGAACCCAACAG |
c72378-RT-R | GCTTCCCGGATTTCTTCTTC | |
c50108-RT-F | Calmodulin (CaM) | CGAGAAGCTTTCCGAGTGTT |
c50108-RT-R | TCCGTCCCCATCTAAATCAG | |
c66278-RT-F | Calmodulin (CaM) | GAGACCGTCGGCATCAGTAT |
c66278-RT-R | TGAAGAAGTATCAGCGGAGGAT | |
c64073-RT-F | astacin | ATCACCGCCATCTGTTCCT |
c64073-RT-R | TCCGCTTCTACTACTTCCTGTA | |
c73606-RT-F | tyr-3 | TGGTCAGGTCTGTAGGTTGTG |
c73606-RT-R | TGGATGTTCTTCGGTGATGGT | |
c51674-RT-R | TCCTCGGATCTTGTCTGACTTC | |
c76659-RT-F | Sulfur quinone oxidoreductase | CGACTACGACGCTAATCCTCT |
c76659-RT-R | ATGATGTTGCCTTCACTCTTCC | |
c75964-RT-F | CYP3A | GCACGGTCCAACCTTAATACTG |
c75964-RT-R | AGGCGACAAGAGATTCAAGAAC | |
c66049-RT-F | CRBP | GATGAAGTCACGGCAGATGG |
c66049-RT-R | CTAGTGATGACCGATTCAGGAT |