From: Multiple stressors produce differential transcriptomic patterns in a stream-dwelling salamander
Experiments (materials and/or conditions) | Group Variables | qPCR N | RNA-Seq N |
---|---|---|---|
Test for differential expression by Temp and Cort exposure over 30 days. (all wild-caught, adult, Paedo) | Control, 11 °C (low) | 8 | 6 |
100 nM Cort, 11 °C (low) | 7 | 6 | |
Control, 21 °C (high) | 7 | 6 | |
100 nM Cort, 21 °C (high) | 8 | 6 | |
Test for differential expression by Cort exposure over 28 days among larvae of alternative life cycle strategies. (lab raised 21 °C, larvae, Paedo & Biphasic) | Control, Paedo (P) | 6 |  |
100 nM Cort, Paedo (P) | 6 |  | |
Control, Biphasic (B) | 6 | Â | |
100 nM Cort, Biphasic (B) | 6 |  | |
Test for differential expression by Cort exposure over time and after regeneration. (lab raised 21 °C, larvae, Biphasic) | Control, 0 day | 6 |  |
100 nM Cort, 14 day | 6 |  | |
100 nM Cort, 28 day | 6 |  | |
100 nM Cort, Regen 14 day | 6 |  | |
Test for differential expression by Cort exposure of tails in culture. (lab raised 21 °C, 48 h, larvae, Paedo) | Control | 6 |  |
100 nM Cort | 6 |  | |
Test for differential expression in stable (21 °C) vs fluctuating (14 to 28 °C) temps with the same mean (21 °C). (all wild-caught, adults, Paedo & Biphasic) | Stable temp, Paedo (P) | 5 |  |
Fluctuating temp, Paedo (P) | 5 | Â | |
Stable temp, Biphasic (B) | 6 | Â | |
Fluctuating temp, Biphasic (B) | 6 | Â |