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Table 1 List of five gene expression experiments included in this study. This list includes the purpose, material, and conditions of the experiment, variables for each experimental group, and samples sizes (N) for qPCR of candidate genes and RNA-Seq

From: Multiple stressors produce differential transcriptomic patterns in a stream-dwelling salamander

Experiments (materials and/or conditions) Group Variables qPCR N RNA-Seq N
Test for differential expression by Temp and Cort exposure over 30 days. (all wild-caught, adult, Paedo) Control, 11 °C (low) 8 6
100 nM Cort, 11 °C (low) 7 6
Control, 21 °C (high) 7 6
100 nM Cort, 21 °C (high) 8 6
Test for differential expression by Cort exposure over 28 days among larvae of alternative life cycle strategies. (lab raised 21 °C, larvae, Paedo & Biphasic) Control, Paedo (P) 6  
100 nM Cort, Paedo (P) 6  
Control, Biphasic (B) 6  
100 nM Cort, Biphasic (B) 6  
Test for differential expression by Cort exposure over time and after regeneration. (lab raised 21 °C, larvae, Biphasic) Control, 0 day 6  
100 nM Cort, 14 day 6  
100 nM Cort, 28 day 6  
100 nM Cort, Regen 14 day 6  
Test for differential expression by Cort exposure of tails in culture. (lab raised 21 °C, 48 h, larvae, Paedo) Control 6  
100 nM Cort 6  
Test for differential expression in stable (21 °C) vs fluctuating (14 to 28 °C) temps with the same mean (21 °C). (all wild-caught, adults, Paedo & Biphasic) Stable temp, Paedo (P) 5  
Fluctuating temp, Paedo (P) 5  
Stable temp, Biphasic (B) 6  
Fluctuating temp, Biphasic (B) 6