Fig. 6

Expression of antiviral genes in A549 cells transfected with small RNA isolated from EVs produced by WNV-infected cells. A549 cells were transfected with small RNA isolated from EVs secreted by WNV-infected or uninfected (mock) cells and gene expression was determined at 24h post transfection by qRT-PCR. Gene expression was calculated by ΔΔCt method relative to the cells transfected with small RNAs from mock EVs and using expression level of housekeeping gene TBP as a reference. Values are the means of 3 biological replicates ±SD. Dash line indicates RNA levels in cells transfected with mock EVs RNA. Statistics analysis was performed using multiple t-tests with Bonferroni correction. ***P<0.001, **P<0.01