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Fig. 1 | BMC Genomics

Fig. 1

From: Global transcriptome profiling and functional analysis reveal that tissue-specific constitutive overexpression of cytochrome P450s confers tolerance to imidacloprid in palm weevils in date palm fields

Fig. 1

Maximum likelihood consensus tree of CYP4 clan. CYP4 clan sequences from Tribolium castaneum, Bemisia tabaci, Leptinotarsa decemlineata and Drosophila melanogaster P450s were used as references to classify R. ferrugineus CYP4 (red). Multiple sequence alignments were performed using MAFFT v.7 with the E-INS-i iterative refinement strategy and default parameters, and the LG + I + G + F substitution model was determined as the best-fit model of protein evolution by ProtTest 3. The maximum likelihood (ML) analysis was computed using PhyML 3.0, with 1000 bootstrap replications and the bootstrap values are indicated at the nodes. P450s from different species were marked with different colors. The phylogenetic tree was visualized using FigTree (http://tree.bio.ed.ac.uk/software/figtree/) and branch appearance was colored based on the bootstrap values. Scale = 0.5 amino acid substitutions per site

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